| 1. | The optimum ph and temperature for the protease activity are ph 10 . 0 and 40 . c . the enzyme is cold - active alkaline serine protease
此菌株为嗜冷嗜碱细菌，经16srrna鉴定，属假单胞菌属。 |
| 2. | The cub domain and serine protease domain 01 clsp closely resembled the corresponding domains of clr ( 48 % and 58 % identity , respectively )
Clsp的cub结构域和丝氨酸蛋白酶结构域与clr相应的结构域之间的相似性( simllarity )分别为为48 %和58 % 。 |
| 3. | All these results demonstrated that dhap is a serine protease . the first 20 amino acid residues of the purified dhap were determined and the sequence is aqtvpygipqikapavhaqg
将纯化的dhap进行转膜，测定了n一末端的加个氨基酸残基的序列，其结果是: aqtvpygipqikap /汉haqg 。 |
| 4. | The masp ( mannose - binding lectin - associated serine protease ) gene has been cloned by the method of degenerative pcr and the fragment of the pcr product is 630 bps in length
本文还利用pcr方法从青岛文昌鱼基因组dna中克隆masp (甘露聚糖结合凝集素相关丝氨酸蛋白酶)基因片段。 |
| 5. | Clr is a complement serine protease , composed of two cub ( clr / cls , an embryonic sea urchin protein uegf , and bone morphogenetic protein i ) domains , one egf domain and one serine protease domain
补体组分c1r含有两个cub结构域，一个egf结构域和一个丝氨酸蛋白酶结构域，是一种丝氨酸蛋白酶原。 |
| 6. | Comparison of the entire derived peptide sequence with other serine protease revealed significant homology , especially with a reported gene from another b . pumilus strain tyo - 67 ( 99 % homology )
将克隆到的碱性蛋白酶基因(命名为ap )编码的成熟蛋白n一端序列与纯化的脱毛蛋白酶n一端序列进行比较，二者完全相同。 |
| 7. | For trypsin - like serine protease domain , the presence of his ( h ) - asp ( d ) - ser ( s ) representing a serine protease catalytic triad were at position of 283 , 339 , 436 , respectively
Clsp在正常人外周血单核细胞来源的dc中有较强的表达，在分别经anti一cd40 、 tnf一a 、 lps刺激后的dc中，其表达水平均有所上升。 |
| 8. | The clsp protein shared roughly 25 - 40 % identity and 40 - 55 % similarity to complement subcomponent clr , cls , mannose - associated serine protease - 1 ( masp - 1 ) , masp - 2 , and masp - 3
通过在genba川k / embl数据库中对clsp序列进行同源性搜索和分析，我们发现其核酸和氨基酸序列和人clr 、 cis 、 maspz 、 maspz 、 masp3等有高度同源性。 |
| 9. | On binding to specific carbohydrate structures on the surface of a broad range of microorganisms , mbl can activate complement system through the activation of mbl - associated serine proteases ( masps ) in antibody - and clq - independent way
甘露聚糖结合凝集素( mannan - bindinglectin ， mbl )是c型凝集素超级家族中胶凝素家族成员。 |
| 10. | The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues , which is part of a alkaline serine protease , with 98 . 3 % homogeneity to a reported gene from bacillus pumilus
序列分析显示，该片段是一个全长1106bp的orf ，可编码369个氨基酸残基的多肽，与短小芽孢杆菌碱性蛋白酶基因编码区高度同源。 |
